A microfluidic fluidized bed to capture, amplify and detect bacteria from raw samples Article - 2018

Lucile Alexandre, Iago Pereiro, Amel Bendali, Sanae Tabnaoui, Jana Srbova, Zuzana Bilkova, Shane Deegan, Lokesh Joshi, Jean-Louis Viovy, Laurent Malaquin, Bruno Dupuy, Stephanie Descroix

Lucile Alexandre, Iago Pereiro, Amel Bendali, Sanae Tabnaoui, Jana Srbova, Zuzana Bilkova, Shane Deegan, Lokesh Joshi, Jean-Louis Viovy, Laurent Malaquin, Bruno Dupuy, Stephanie Descroix, « A microfluidic fluidized bed to capture, amplify and detect bacteria from raw samples  », Methods in Cell Biology, numéro spécial Microfluidics in Cell Biology Part B : Microfluidics in Single Cells, 2018, pp. 59-75. ISSN 0091-679X

Abstract

Bacterial contamination and subsequent infections are a major threat to human health. An early detection in the food chain, clinics or the environment, is key to limit this threat. We present a new concept to develop low-cost hand-held devices for the ultra-sensitive and specific detection of bacteria in a one-step process of 2–8 h, directly from complex raw samples. This approach is based on a novel microfluidic magnetic fluidized bed. It reaches a 4 CFU (colony forming unit) sensitivity with high quantification accuracy in a large dynamic range of 100–107CFU/mL. The versatility of the approach was demonstrated with the detection of different bacteria strains, among which Salmonella Typhimurium and E. coli O157:H15. Additionally, the method is sensitive to infectious bacteria only, a criterion requested by main applications and currently requiring additional culture steps of one to several days.

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