Blood schizontocidal activity of methylene blue in combination with antimalarials againstPlasmodium falciparum Article - Juin 2007

Giovanny Garavito, Stephane Bertani, Javier Rincon, Séverine Maurel, Marie-Carmen Monje, Irene Landau, Alexis Valentin, Eric Deharo

Giovanny Garavito, Stephane Bertani, Javier Rincon, Séverine Maurel, Marie-Carmen Monje, Irene Landau, Alexis Valentin, Eric Deharo, « Blood schizontocidal activity of methylene blue in combination with antimalarials againstPlasmodium falciparum  », Parasite, juin 2007, pp. 135-140. ISSN 1776-1042

Abstract

Methylene blue (MB) is the oldest synthetic antimalarial. It is not used anymore as antimalarial but should be reconsidered. For this purpose we have measured its impact on both chloroquine sensitive and resistant Plasmodium strains. We showed that around 5 nM of MB were able to inhibit 50% of the parasite growth in vitro and that late rings and early trophozoites were the most sensitive stages ; while early rings, late trophozoites and schizonts were less sensitive. Drug interaction study following fractional inhibitory concentrations (FIC) method showed antagonism with amodiaquine, atovaquone, doxycycline, pyrimethamine ; additivity with artemether, chloroquine, mefloquine, primaquine and synergy with quinine. These results confirmed the interest of MB that could be integrated in a new low cost antimalarial combination therapy.

Voir la notice complète sur HAL

Actualités