No evidence for viral sequences in five lepidic adenocarcinomas (former “BAC”) by a high-throughput sequencing approach Article - Décembre 2015

Nicolas Berthet, L. Frangeul, Ken André Olaussen, Elisabeth Brambilla, Nicolas Dorvault, Philippe Girard, Pierre Validire, Elie Fadel, Christiane Bouchier, Antoine Gessain, Jean-Charles Soria

Nicolas Berthet, L. Frangeul, Ken André Olaussen, Elisabeth Brambilla, Nicolas Dorvault, Philippe Girard, Pierre Validire, Elie Fadel, Christiane Bouchier, Antoine Gessain, Jean-Charles Soria, « No evidence for viral sequences in five lepidic adenocarcinomas (former “BAC”) by a high-throughput sequencing approach  », BMC Research Notes, décembre 2015, p. 782. ISSN 1756-0500

Abstract

Background The hypothesis of an infectious etiology of the formerly named bronchiolo-alveolar carcinoma (BAC) has raised controversy. We investigated tumor lung tissues from five patients with former BAC histology using high-throughput sequencing technologies to discover potential viruses present in this type of lung cancer. Around 180 million single reads of 100 bases were generated for each BAC sample. Results None of the reads showed a significant similarity for Jaagsiekte sheep retrovirus (JSRV) and no other viruses were found except for endogenous retroviruses. Conclusions In conclusion, we have demonstrated the absence of JSRV and other known human viruses in five samples of well-characterized lepidic adenocarcinoma. Background The bronchiolar-alveolar cancer (BAC) in its past definition (WHO classification 1999) is a rare form of lung adenocarcinoma (ADC). The international WHO 2015 classification recommends distinguishing adenocarcinoma in situ (AIS, formerly non-mucinous BAC) from invasive mucinous adenocarcinoma (IMA, formerly mucinous BAC) and non-mucinous lepidic predominant invasive adenocarcinoma of the lungs [1]. In many such patients, the tumor progression respects the pulmonary architecture and develops mainly in the terminal respiratory unit (lepidic growth).

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