Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps Article - Décembre 2012

Alice Verchère, Isabelle Broutin, Martin Picard

Alice Verchère, Isabelle Broutin, Martin Picard, « Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps  », Scientific Reports, décembre 2012. ISSN 2045-2322

Abstract

We describe an original activity assay for membrane transport that uses the proton motive force-dependent efflux pump MexAB from Pseudomonas aeruginosa. This pump is co-reconstituted into proteoliposomes together with bacteriorhodopsin (BR), a light-activated proton pump. In this system, upon illumination with visible light, the photo-induced proton gradient created by the BR is shown to be coupled to the active transport of substrates through the pump.

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