Self-assembled monolayer for AFM measurements of Tobacco Mosaic Virus (TMV) at the atomic level Article - Février 2014

Matthieu Meillan, Michaël Ramin, Thierry Buffeteau, Sophie Marsaudon, Michaël Odorico, Shu-Wen Chen, Jean-Luc Pellequer, Marie Degueil, Karine Heuzé, Luc Vellutini, Bernard Bennetau

Matthieu Meillan, Michaël Ramin, Thierry Buffeteau, Sophie Marsaudon, Michaël Odorico, Shu-Wen Chen, Jean-Luc Pellequer, Marie Degueil, Karine Heuzé, Luc Vellutini, Bernard Bennetau, « Self-assembled monolayer for AFM measurements of Tobacco Mosaic Virus (TMV) at the atomic level  », RSC Advances, février 2014, p. 11927. ISSN 2046-2069

Abstract

Biosensors are based on the conversion of a biological response to an electrical signal. One of the major challenges is to ascertain that the receptor is not denatured when immobilised (covalently or not) on the device. In this work, a protein receptor (virus) was immobilised on two different surfaces, mica and self-assembled monolayer (SAM), and its height was determined by atomic force microscopy measurements at the atomic level. Results clearly showed that expected dimensions of Tobacco Mosaic Virus (TMV) are obtained after immobilisation onto a soft organic SAM.

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