Terbium(III) Luminescent Complexes as Millisecond-Scale Viscosity Probes for Lifetime Imaging Article - Juin 2017

Anh Thy Bui, Alexei Grichine, Alain Duperray, Pierre Lidon, François Riobé, Chantal Andraud, Olivier Maury

Anh Thy Bui, Alexei Grichine, Alain Duperray, Pierre Lidon, François Riobé, Chantal Andraud, Olivier Maury, « Terbium(III) Luminescent Complexes as Millisecond-Scale Viscosity Probes for Lifetime Imaging  », Journal of the American Chemical Society, juin 2017, pp. 7693 - 7696. ISSN 0002-7863

Abstract

Fluorescent probes that are able to directly measure viscosity are attractive candidates for the study of intracellular environments. We report a new class of luminescent rotors, based on the sensitized emission of a terbium(III) complex. A 4-fold increase in both quantum yield and luminescence lifetime was observed in viscous media for the studied complexes, with a lifetime ranging from 0.23 to 0.89 ms over a broad range of viscosities (0.6–1200 cP). The presented approach, relying on the millisecond-scale luminescence lifetime of the lanthanide ions, was applied to fixed T24 cancer cells using temporal sampling lifetime imaging microscopy.

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