Unveiling the Binding Modes of the Crystallophore, a Terbium-based Nucleating and Phasing Molecular Agent for Protein Crystallography Article - Mai 2018

Sylvain Engilberge, François Riobé, Tristan Wagner, Sebastiano Di Pietro, Cécile Breyton, Bruno Franzetti, Seigo Shima, Eric Girard, Elise Dumont, Olivier Maury

Sylvain Engilberge, François Riobé, Tristan Wagner, Sebastiano Di Pietro, Cécile Breyton, Bruno Franzetti, Seigo Shima, Eric Girard, Elise Dumont, Olivier Maury, « Unveiling the Binding Modes of the Crystallophore, a Terbium-based Nucleating and Phasing Molecular Agent for Protein Crystallography  », Chemistry - A European Journal, mai 2018, pp. 9739-9746. ISSN 0947-6539

Abstract

Crystallophores are lanthanide complexes that act as powerful auxiliary for protein crystallography due to their strong nucleating and phasing effects. To get first insights on the mechanisms behind nucleation induced by Crystallophore, we systematically identified various elaborated networks of supramolecular interactions between Tb-Xo4 and subset of 6 protein structures determined by X-ray diffraction in complex with terbium-Crystallophore (Tb-Xo4). Such interaction mapping analyses demonstrate the versatile binding behavior of the Crystallophore and pave the way to a better understanding of its unique properties.

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