Zero-Mode Waveguide Detection of Flow-Driven DNA Translocation through Nanopores Article - Juillet 2014

Thomas Auger, Jérôme Mathé, Virgile Viasnoff, Gaëlle Charron, Jean-Marc Di Meglio, Loïc Auvray, Fabien Montel

Thomas Auger, Jérôme Mathé, Virgile Viasnoff, Gaëlle Charron, Jean-Marc Di Meglio, Loïc Auvray, Fabien Montel, « Zero-Mode Waveguide Detection of Flow-Driven DNA Translocation through Nanopores  », Physical Review Letters, juillet 2014, p. 028302. ISSN 0031-9007

Abstract

We directly measure the flow-driven injection of DNA through nanopores at the level of single molecule and single pore using a modified zero-mode waveguide method. We observe a flow threshold independent of the pore radius, the DNA concentration, and length. We demonstrate that the flow injection of DNA in nanopores is controlled by an energy barrier as proposed in the de Gennes-Brochard suction model. Finally, we show that the height of the energy barrier is modulated by functionalizing the nanopores.

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